rat-anti-mouse mac2 primary antibody  (Cedarlane)

Journal: bioRxiv

Article Title: IL-18 inhibition enlarges lesions, necrotic cores and thickens fibrous caps in Jak2 V617F clonal hematopoiesis-driven atherosclerosis

doi: 10.1101/2025.06.03.657754

Figure Lengend Snippet: A. UMAP of integrated single-cell RNA-sequencing of CD45+ plaque cells isolated from aortic arches of control mice, Jak2 VF mice, and Jak2 VF mice with IL-18 antibodies. B. Percentage of lesional CD45 + cells within each cluster. C. Violin plots depicting gene expression levels of efferocytosis markers Axl , Mertk , and Cd36 in control, Jak2 VF , and Jak2 VF mice treated with IL-18 antibody, derived from Sc-RNA-seq data. D. Representative aortic images with DAPI, MAC2, and AXL staining. DAPI is shown in blue, MAC2 is shown in green, and AXL is shown in magenta. AXL positive macrophages (white spots) are highlighted by yellow arrows in the lesions. E. Quantification of lesional AXL positive macrophages (n=15/group) after 10 weeks of WTD feeding. F. Violin plots depicting gene expression levels of MHC II genes in Jak2 VF , and Jak2 VF mice treated with IL-18 antibody, derived from Sc-RNA-seq data. Scale bar, 100 μm. Statistical analyses were performed using the Wilcoxon Rank Sum test with Bonferroni correction in C. For E statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison test. Statistical differences are indicated on the graphs, with p-values greater than 0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs.

Article Snippet: Samples were immune stained with TUNEL reagent (Roche), and anti-Mac2 antibodies (Cedarlane).

Techniques: RNA Sequencing, Isolation, Control, Gene Expression, Derivative Assay, Staining, Comparison

Journal: Frontiers in Pharmacology

Article Title: Clusterin ameliorates diabetic atherosclerosis by suppressing macrophage pyroptosis and activation

doi: 10.3389/fphar.2025.1536132

Figure Lengend Snippet: Clu suppressed macrophages pyroptosis in vivo . (A) Representative transmission electron microscopy images of atherosclerotic lesions in ApoE −/− and ApoE −/− Clu −/− mice. Scale bar: 1 μm. (B) Representative images of immunofluorescence staining for MAC2 and NLRP3 in atherosclerotic lesions of ApoE −/− and ApoE −/− Clu −/− . Scale bar: 0.050 mm. (C) Representative images of immunofluorescence staining for F4/80 and NLRP3 in atherosclerotic lesions of mice injected with AAV-NC or AAV- Clu . Scale bar: 20 μm. (D) Western blotting for NLRP3, caspase-1, and IL-1β in mice injected with AAV-NC or AAV- Clu . (E) Relative quantification of the NLRP3 expression. (F) Relative quantification of the caspase-1 expression. (G) Relative quantification of the IL-1β expression. DKO, ApoE −/− Clu −/− . n = 4 per group. *** P < 0.001.

Article Snippet: Anti-MAC2 antibody was from Servicebio (Wuhan, China).

Techniques: In Vivo, Transmission Assay, Electron Microscopy, Immunofluorescence, Staining, Injection, Western Blot, Quantitative Proteomics, Expressing

Journal: iScience

Article Title: Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice

doi: 10.1016/j.isci.2025.111903

Figure Lengend Snippet: STEAP4 is highly expressed in adipose tissue and mature adipocytes, and its deficiency in adipocytes promotes HFD-induced obesity (A) Bulk tissue gene expression for STEAP4 in human tissues reported by GTEx. (B) Steap4 expression measured in selected mouse tissues by western blot. (C) Steap4 expressions analyzed in isolated SVF (stromal vascular fraction) or mature adipocytes by western blot. (D) Body weight of Steap4 f/f and Steap4 AKO mice fed an HFD diet for 12 weeks ( n = 7, 6). (E) Indicated organs weight from HFD-fed Steap4 f/f and Steap4 AKO mice after 12 weeks HFD feeding ( n = 6 pairs). (F) EchoMRI analysis of fat mass and lean mass from Steap4 f/f and Steap4 AKO mice after HFD feeding ( n = 7, 6). (G–H) Glucose tolerance test (GTT) and insulin tolerance test (ITT) in HFD-fed Steap4 f/f and Steap4 AKO mice ( n = 7, 6). (I) Upper: Representative images (20x) of Hematoxylin and Eosin (H&E) staining and Mac2 staining of iWAT sections from HFD-fed Steap4 f/f and Steap4 AKO mice. Scale bars: 150 μm. Lower: Quantification of crown-like structure (CLS) numbers in 10x objective ( n = 12 fields). (J) Left: Representative images of H&E staining and Ucp1 staining of iBAT sections from HFD-fed Steap4 f/f and Steap4 AKO mice. Right: Quantification of relative Ucp1 + area by ImageJ Software ( n = 12 fields). Scale bars: 150 μm (H&E) and 75 μm (Ucp1). (K) Expression of the indicated mRNAs in iBAT from HFD-fed Steap4 f/f and Steap4 AKO mice ( n = 6 each group). Statistical significance for all relevant graphs was determined by two-tailed Student’s test. Significance: ∗ 0.01 ≤ p < 0.05, ∗∗ 0.001 ≤ p < 0.01, ∗∗∗ p < 0.001; ns, not significant. All data represent means ± SEM.

Article Snippet: Sections on slides were further blocked with 3 % BSA in PBS solution for at least 30 min. Anti-Mac2 monoclonal antibody (Cedarlane, CL8942AP; 0.5 μg/ml) or anti-UCP1 monoclonal antibody (Abcam, ab261912, dilution 1: 50) was incubated at 4°C overnight.

Techniques: Gene Expression, Expressing, Western Blot, Isolation, Staining, Software, Two Tailed Test

Journal: iScience

Article Title: Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice

doi: 10.1016/j.isci.2025.111903

Figure Lengend Snippet:

Article Snippet: Sections on slides were further blocked with 3 % BSA in PBS solution for at least 30 min. Anti-Mac2 monoclonal antibody (Cedarlane, CL8942AP; 0.5 μg/ml) or anti-UCP1 monoclonal antibody (Abcam, ab261912, dilution 1: 50) was incubated at 4°C overnight.

Techniques: Recombinant, SYBR Green Assay, Real-time Polymerase Chain Reaction, Reverse Transcription, Isolation, BIA-KA, RNA Sequencing, Mass Spectrometry, Expressing, Alternative Splicing, Software, Functional Assay